Reconstitution of Fully Active RNase S by Carboxypeptidase-degraded RNase S-Peptide
نویسندگان
چکیده
منابع مشابه
Reconstitution of fully active RNase S by carboxypeptidase-degraded RNase S-peptide.
In attempting to relate the covalent structure of ribonuclease to its enzymic activity, controlled proteolytic degradation is a useful approach. Although native ribonuclease is markedly resistant to a variety of proteolytic enzymes, the separated components of ribonuclease S (1, 2), S-peptide, and S-protein, are extensively degraded by carboxypeptidase. Previous studies (3) on the sequence of a...
متن کاملIdentification of Self- incompatibility Alleles in Some Almond Genotypes by Degenerate S-RNase Primers
The almond, Prunus dulcis Miller which belongs to Rosaceae family, is one of the most important commercial and oldest cultivated tree nut crops. Almonds are classified as a ‘nut’ in which the edible seed is the commercial product. Therefore, pollination and fertilization are necessary in almond. The characteristic of cultivated almond to express gametophytic self- incompatibility discourages se...
متن کاملS-RNase-mediated self-incompatibility.
The Solanaceae, Rosaceae, and Scrophulariaceae families all possess an RNase-mediated self-incompatibility mechanism through which their pistils can recognize and reject self-pollen to prevent inbreeding. The highly polymorphic S-locus controls the self-incompatibility interaction, and the S-locus of the Solanaceae has been shown to be a multi-gene complex in excess of 1.3 Mb. To date, the func...
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Gametophytic self-incompatibility (GSI) allows plants to block fertilization by haploid pollen whose S-allele constitution matches one of the two S-alleles in the diploid styles. GSI in Solanum chacoense requires a stylar S-RNase, first secreted from cells of the transmitting tract then imported into incompatible (self) pollen tubes. However, the molecular mechanisms allowing compatible pollen ...
متن کاملS-RNase complexes and pollen rejection.
Biochemical interactions between the pollen and the pistil allow plants fine control over fertilization. S-RNase-based pollen rejection is among the most widespread and best understood of these interactions. At least three plant families have S-RNase-based self-incompatibility (SI) systems, and S-RNases have also been implicated in interspecific pollen rejection. Although S-RNases determine the...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1963
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)68012-9